![]() ( C) Plasma was pre-incubated with different amounts of CM (as indicated, 0, 25, 50, or 100 nmol/L) followed by thrombin-initiated clot formation (as above), and the clot was lysed in the presence of 100 ng/mL tPA. Each value represents the mean of triplicate determinations. ) at different tPA levels or control buffer (○).( B) The plasma clot lysis time (t 1/2, min for 50 % clot lysis) was quantified in the presence of CM ( Following rapid mixing, the reaction mixture was monitored for turbidity at 405 nm at 37☌ for 180 min during which a clot was rapidly formed (turbidity increase) and then that clot was subsequently lysed (turbidity decrease). ( A) As described in Materials and Methods, a 100 μL aliquot containing thrombin, Ca 2+, phospholipid vesicles (60% PC: 20% PS: 20% PE), 0.1% BSA and different levels of tPA (as indicated, 0, 50, 100, or 200 ng/mL) was added to a 100 μL aliquot containing citrated normal human pooled plasma which had been pre-incubated for 30 min at 37 ☌ with CM (100 nmol/L) (solid lines) or control buffer (dotted lines). Future work is needed to clarify CM's pathophysiology and its mechanistic influences on hemostasis or thrombosis.Ĭardiac myosins fibrin hemostasis thrombin thrombosis. CM's procoagulant and antifibrinolytic activities likely involve, at least in part, its ability to bind factor Xa and enhance thrombin generation. ![]() CM in vivo can augment myocardial damage and can be prohemostatic in the presence of bleeding. In tPA (tissue-type plasminogen activator)-induced plasma clot lysis assays, CM was antifibrinolytic due to robust CM-dependent thrombin generation that enhanced activation of TAFI (thrombin activatable fibrinolysis inhibitor).ĬM in vitro is procoagulant and prothrombotic. Binding studies showed that CM, like skeletal muscle myosin, directly binds factor Xa, supporting the concept that the CM surface is a site for prothrombinase assembly. Thrombin generation assays showed that CM, like skeletal muscle myosin, enhanced thrombin generation in human platelet-rich and platelet-poor plasmas and also in mixtures of purified factors Xa, Va, and prothrombin. In vitro studies clarified some mechanisms for CM's procoagulant properties. These data provide proof of concept for CM's in vivo procoagulant properties. ![]() In hemophilia A mice, intravenously administered CM reduced tail-cut-initiated bleeding. In a murine ischemia/reperfusion injury model, exogenous CM, when administered intravenously, augmented myocardial infarction and troponin I release. Addition of CM to blood passing over collagen-coated surfaces enhanced fibrin formation. Approach and Results: Perfusion of fresh human blood over CM-coated surfaces caused thrombus formation and fibrin deposition. ![]() Here, we evaluated CM's ex vivo, in vivo, and in vitro activities related to hemostasis and thrombosis. Gupta had registered an FIR in this connection at Jamshedpur.Cardiac myosin (CM) is structurally similar to skeletal muscle myosin, which has procoagulant activity. Spokesperson Singh said that the public is also looking towards the state Congress to see what action the party takes against the party colleague sitting on such a responsible post. The spokesperson also questioned the silence of senior congress leader of the party Rahul Gandhi saying that ‘the crown prince of the Congress party, Mr Rahul Gandhi, does not even have a tweet in this regard, exposes the cunning character of the Congress.’ Ranchi, April 24: BJP sought clarification from CM Hemant Soren and Congress party leaders on the viral video clip showing state health minister Gupta engaged in a sexual video chat with a woman.īJP state spokesperson Saroj Singh said that it was expected from CM Soren to clarify as the concerned minister is his senior cabinet colleague and Congress should speak on the issue as the concerned person is one of the four congress party legislatures in the cabinet of the JMM led UPA government.
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